Composite

Part:BBa_K734004:Design

Designed by: Razan Alnahhas   Group: iGEM12_Austin_Texas   (2012-09-25)

sfGFP regulated by LasR quorum sensing pathway


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5486
    Illegal PstI site found at 3199
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2412
    Illegal NheI site found at 4644
    Illegal PstI site found at 3199
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3240
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5486
    Illegal PstI site found at 3199
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5486
    Illegal PstI site found at 3199
    Illegal NgoMIV site found at 1898
    Illegal AgeI site found at 1701
    Illegal AgeI site found at 3462
    Illegal AgeI site found at 4730
    Illegal AgeI site found at 5054
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3367


Design Notes

The plasmid design entails putting together 6 parts from different plasmids and a plasmid backbone, so the construction took time and needed high concentrations of each part.


Source

Parts were obtained from iGEM Registry, and pACYC, PQL269, and pET21 plasmids.

References